Spatial distribution of cannabinoid content within a plant

Spatial distribution of cannabinoid content within a plant
Photo: Cultivators

Cultivators and Brightlabs are both members of the in the Cultivation for Compounds (CfC) consortium. The consortium, consisting of large companies within the horticultural industry, was founded to facilitate research on the cultivation of medical cannabis and its beneficial compounds.

The CfC has found a reliable and consistent partner in Brightlabs for its analyses on the quality of the produced flower. In the CfC’s independently developed sampling protocols, a mixed sample is collected from all parts of the plant before the sample is sent for analysis. To verify the correctness of the protocol for our cultivation set-up, questions arose regarding the distribution of cannabinoids through different parts of the plant.  As it is known that cannabinoids levels can differ depending on the position of the flower, cultivar, lightning and many other variables. Therefore it is of importance to get insight into these differences so they can take into account.

Goal:
  • Verify the sampling protocol developed for CfC’s specific cultivation set-up
Experimental setup:

A representative plant from the reference treatment from the winter 2023 research cultivation cycle was selected for furtheranalysis. The plant was grown in a high-tech greenhouse in the Netherlands, where the CfC consortium performs the experiments. The greenhouse is equipped with a gutter cultivation system, pressure compensated drip irrigation, and an active climate control system including CO2 supplementation. A dynamic LED system ensured a consistent light intensity of 1000 µmol/m2/s on the crop. The reference treatment was grown on stone wool.

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