The earliest sign for twospotted spider mite is knowing your crop

"We tell people to try to find the adults because the two spots are an accumulation of the body waste, so in the earlier stages it may not be as clear whether they are there or not," Leavitt says. "That being said, we've all seen the clear spider mites with those two black spots, which is great, but the spots can also be orange, different shades of red, yellow, etc. due to their different environments. Don't be too quick to judge on color."

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Source: Greenhouse Grower